Strain for producing citrulline and method for biologically synthesizing citrulline with same



The invention provides a strain for producing citrulline and a method for biologically synthesizing citrulline with the same, belonging to the food biotechnology field. The strain and the method have the following beneficial effects: the invention relates to enterococcus faecalis (SK23.001) screened from the soil; enterococcus faecalis has been collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:M2011465; the strain is taken as the fermentation strain, arginine is taken as the inducer, glucose is taken as the carbon source and yeast cream and peptone are taken as nitrogen sources to form a fermentation medium to ferment citrulline or a resting cell thallus with arginine deiminase activity, and the thallus is added to 5-20% of arginine solution to synthesize citrulline through transformation; through detection, the transformation rate can be more than 80%; citrulline produced by the method is safe and reliable, is a functional product with great market potential and is widely applied to such industries as food, cosmetics, medicines and the like; and the method can be used for efficiently producing citrulline and is suitable for large scale production.




Download Full PDF Version (Non-Commercial Use)

Patent Citations (4)

    Publication numberPublication dateAssigneeTitle
    CN-100478440-CApril 15, 2009张 鹏;张淑荣;刘春巧Method of immobilized cell continuously producing L-carbamyl ornithine
    CN-100584940-CJanuary 27, 2010江南大学Strain for producing arginine deiminase and application thereof
    CN-102220390-AOctober 19, 2011上海聚瑞生物技术有限公司Method for preparing citrulline by combining arginine fermentation and enzymatic conversion
    CN-1189560-AAugust 05, 1998吕清录运动场渗透式无障碍排水装置

NO-Patent Citations (0)


Cited By (0)

    Publication numberPublication dateAssigneeTitle