Strain for producing citrulline and method for biologically synthesizing citrulline with same

一株产瓜氨酸的菌株及用该菌株生物合成瓜氨酸的方法

Abstract

The invention provides a strain for producing citrulline and a method for biologically synthesizing citrulline with the same, belonging to the food biotechnology field. The strain and the method have the following beneficial effects: the invention relates to enterococcus faecalis (SK23.001) screened from the soil; enterococcus faecalis has been collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:M2011465; the strain is taken as the fermentation strain, arginine is taken as the inducer, glucose is taken as the carbon source and yeast cream and peptone are taken as nitrogen sources to form a fermentation medium to ferment citrulline or a resting cell thallus with arginine deiminase activity, and the thallus is added to 5-20% of arginine solution to synthesize citrulline through transformation; through detection, the transformation rate can be more than 80%; citrulline produced by the method is safe and reliable, is a functional product with great market potential and is widely applied to such industries as food, cosmetics, medicines and the like; and the method can be used for efficiently producing citrulline and is suitable for large scale production.
一株产瓜氨酸的菌株及用该菌株生物合成瓜氨酸的方法,属于食品生物技术领域。本发明涉及一株从土壤中筛选而来的粪肠球菌(Enterococcusfaecalis)SK23.001,已保藏于中国典型培养物保藏中心,保藏编号为CCTCCNO:M2011465,以此菌为发酵菌株,以精氨酸为诱导物、以葡萄糖为碳源、酵母膏和蛋白胨为氮源等组成发酵培养基,发酵生产瓜氨酸,或通过发酵获得具有精氨酸脱亚氨酶酶活的静息细胞菌体并添加到5%-20%精氨酸溶液中转化合成瓜氨酸,经检测可知,转化率可达80%以上。本发明方法生产的瓜氨酸安全可靠,是一种很有市场潜力的功能性产品,被广泛的应用于食品、化妆品、医药等行业。本发明能高效地生产瓜氨酸,适于进行大规模生产。

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